Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech VLP Factory™

Intelligent DNA delivery systems

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VLP Factory™

Technology Description

Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome, making them non-infectious and safe to handle. VLPs are becoming an increasingly widespread tool for the development of vaccines, and are finding other important applications in biotechnology including serving as a display system for integral membrane proteins for antibody screening, production of  immunogens, and for ligand binding assays. The expression of viral structural proteins, such as Matrix proteins, can result in the self-assembly of VLPs.

 

Figure 1. Assembly of VLPs (left and middle). Electron Micrographs of pentavalent Flu VLP Vaccine Candidate Created  Using VLP Factory™ (right).

VLP Factory™ is a baculovirus expression system which constitutively secretes promiscuous virus matrix proteins, with built in functionality to allow you to introduce multiple additional expression cassettes for production of multiprotein VLPs displaying your surface protein, or integral membrane protein, of choice.

VLP Factory™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The VLP Factory™ baculovirus genome (DH10VLPFactory) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots.

VLP Factory Expression System Schematic Diagram

How VLP Factory™ Works:

  • 1) Design and clone gene(s) of interest (GOIs) into MultiBac™ acceptor and donor vectors
  • 2) Match and mix your GOIs and then (re)combine them into one construct
  • 3) Select your construct using unique combination of antibiotic resistance markers
  • 4) Transfer the entire GOIs-assembly into the MultiBac™ bacmid
  • 5) Select and amplify your gene-containing MultiBac™ bacmid
  • 6) Transfect insect cells with purified MultiBac™ bacmid
  • 7) Expression of protein(s) in insect cells

Applications for multiprotein VLPs include their use directly as as multiepitope vaccines, for ligand binding assays, as templates for small molecule drug development, as templates for therapeutic antibody development via phage or yeast display, for screening for and characterizing MAbs, as carriers for membrane proteins e.g. GPCRs or ion channels, as adjuvants or for prime-boost regimens, and as scaffolds for synthetic metabolic pathways (substrate channeling).

References and Downloads

Click here to download VLP Factory™ Manual v1.1

Click here to download Cre-ACEMBLER User Manual v2.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows (32bit)

Click here to download Cre-ACEMBLER for Linux

Kits

VLP Factory™ Expression System Kit 1,149.00€ ENQUIRY

Cell Lines

DH10VLP Factory 819.00€ ENQUIRY
PirHC 279.00€ ENQUIRY
PirLC 279.00€ ENQUIRY

Plasmids

pACEBac1 (5µg) 279.00€ ENQUIRY
pACEBac2 (5µg) 279.00€ ENQUIRY
pIDK (5µg) 279.00€ ENQUIRY
pIDC (5µg) 279.00€ ENQUIRY

Dual Mammalian Insect Cell Plasmids

pFL-IM (5µg) 419.00€ ENQUIRY
pKL-IM (5µg) 419.00€ ENQUIRY
pSPL-IM (5µg) 419.00€ ENQUIRY
pUCDM-IM (5µg) 419.00€ ENQUIRY

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