Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech MultiMam™ Transient

Intelligent DNA delivery systems

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MultiMam™ Transient

Technology Description

Co-Expression of Multiple Flourescent Proteins in One Mammalian Cell

The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. MultiMam™ is a novel and highly efficient modular plasmid-based eukaryotic expression system.

MultiLabel System

A specific subset of transfer vectors called “MutiLabel” were developed by Kriz et al (see reference download link below) and allow scientists to precisely label many cellular components involved in a given disease process and follow them all at the same time. A demonstration of these vectors in action is shown here Link

MultiMam™ Transient and MultiLabel transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.

Schematic Diagram of MultiMam Transient Heterologous Protein Expression System

How MultiMam™ Transient Works:

  • 1) Design and clone your gene(s) of interest (GOIs) into MultiMam™ Transient acceptor and donor vectors
  • 2) Match and mix your GOIs and then (re)combine them into one construct
  • 3) Select your construct using unique combination of antibiotic markers
  • 4) Transfect mammalian cells with composite GOI vector. Transient expression follows.
  • 5) Drug discovery, bioproduction, stem cell differentiation etc.

References and Downloads:

Click here to download Kriz et al 2010

Click here to download MultiMam™ Transient Manual v3.1

Click here to download Cre-ACEMBLER User Manual v2.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows (32bit)

Click here to download Cre-ACEMBLER for Linux

Kits

MultiMam™ Transient Kit 1,149.00€ ENQUIRY

Plasmids

pACEMam1 (5µg) 279.00€ ENQUIRY
pACEMam2 (5µg) 279.00€ ENQUIRY
pMDC (5µg) 279.00€ ENQUIRY
pMDK (5µg) 279.00€ ENQUIRY
pMDS (5µg) 279.00€ ENQUIRY

Cell Lines

PirHC 279.00€ ENQUIRY
PirLC 279.00€ ENQUIRY

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E.coli cell expression
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