LOCUS Exported 5450 bp ds-DNA circular SYN 14-APR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pFL.gb SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5450) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Apr 14, 2017 from SnapGene Viewer 3.2.0 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5450 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 102..556 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 582..686 /gene="bla" /note="AmpR promoter" CDS 687..1547 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1718..2306 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element 2611..2835 /mobile_element_type="transposon:Tn7" /note="Tn7R" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2902..3435) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3624..3652) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" protein_bind 4048..4081 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 4271..4319 /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter complement(4455..4564) /note="p10 promoter" /note="baculovirus promoter for expression in insect cells" promoter 4609..4700 /gene="polh from Autographa californica" /note="polyhedrin promoter" /note="promoter for the baculovirus polyhedrin gene" polyA_signal 4959..5093 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" mobile_element 5203..5368 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga 421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata 541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc 961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat 1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg 2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc 3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc 3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 3961 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca 4021 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta 4081 tctggtttaa acgtacccgt agtggctatg gcagggcttg ccgccccgac gttggctgcg 4141 agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga aacgcgggcg 4201 tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc 4261 ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt 4321 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct 4381 cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc cgggtgatca 4441 agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt taattaatat 4501 acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa 4561 ggtccgtata ctagtatcga ttcgcgacct actccggaat attaatagat catggagata 4621 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg 4681 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat 4741 cccggtccga agcgcgcgga attcaaaggc ctacgtcgac gagctcacta gtcgcggccg 4801 ctttcgaatc tagagcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa 4861 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 4921 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 4981 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 5041 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac 5101 tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct 5161 gccaccgctg agcaataact atcataaccc ctaggagatc cgaaccagat aagtgaaatc 5221 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt 5281 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc 5341 cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa 5401 tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt //