LOCUS       Exported                2312 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(30..63)
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      116..472
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(864..1523)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1524..1626)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     polyA_signal    1798..1932
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2191..2282)
                     /gene="polh from Autographa californica"
                     /label=polh from Autographa californica promoter
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
ORIGIN
        1 gatactagta aaacccatgt gcctggcaga taacttcgta taatgtatgc tatacgaagt
       61 tatggtaccg cggccgcgta gaggatctgt tgatcagcag ttcaacctgt tgatagtacg
      121 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
      181 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
      241 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
      301 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
      361 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
      421 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga cagaattagc
      481 ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa
      541 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctgggagg cagaataaat
      601 gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt
      661 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
      721 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
      781 tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg cgtttaaggg
      841 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt
      901 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      961 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1021 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1081 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1141 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1201 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1261 acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca
     1321 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1381 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1441 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1501 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     1561 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaccc tcttacgtgc
     1621 cgatcaacgt ctcattttcg ccaaaagttg gcccagatct atgtcgggtg cggagaaaga
     1681 ggtaatgaaa tggcacctag gggttatgat agttattgct cagcggtggc agcagccaac
     1741 tcagcttcct ttcgggcttt gttagcagcc ggatcttcta ggctcaagca gtgatcagat
     1801 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1861 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1921 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1981 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     2041 ctagtacttc tcgacaagct tgtcgagact gcaggctcta gattcgaaag cggccgcgac
     2101 tagtgagctc gtcgacgtag gcctttgaat tccgcgcgct tcggaccggg atccgcgccc
     2161 gatggtggga cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt
     2221 acgaaaacag taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg
     2281 atctattaat attccggagt aggtcgcgaa tc
//
