LOCUS       Exported                2281 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        15..124
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     polyA_signal    260..308
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(510..543)
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      545..901
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1262..2056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=aph(3')-II (or nptII)
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gatactagta tacggacctt taattcaacc caacacaata tattatagtt aaataagaat
       61 tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac
      121 aatcactcga cgaagacttg atcacccggg atctcgagcc atggtgctag cagctgatgc
      181 atagcatgcg gtaccgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
      241 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
      301 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
      361 accccattgg gaccaatacg cccgcgtttc ttccttttcc ccaccccaac ccccaagttc
      421 gggtgaaggc ccagggctcg cagccaacgt cggggcggca agccctgcca tagccactac
      481 gggtacgttt aaacccatgt gcctggcaga taacttcgta taatgtatgc tatacgaagt
      541 tatggtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
      601 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
      661 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
      721 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
      781 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
      841 agaagccctt agagcctctc aaagcaattt tcagtgacac aggaacactt aacggctgac
      901 agaattagct tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa
      961 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     1021 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     1081 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     1141 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     1201 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     1261 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1321 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1381 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1441 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1501 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1561 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1621 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1681 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1741 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     1801 cggcgaggat ctcgtcgtga cacatggcga tgcctgcttg ccgaatatca tggtggaaaa
     1861 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     1921 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     1981 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     2041 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     2101 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     2161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     2221 gcccaccccg ggatctatgt cgggtgcgga gaaagaggta atgaaatggc acctaggtat
     2281 c
//
