LOCUS Exported 2231 bp ds-DNA circular SYN 19-MAR-2019 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2231) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 19, 2019 from SnapGene Viewer 4.1.9 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2231 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 16..125 /note="p10 promoter" /note="baculovirus promoter for expression in insect cells" polyA_signal 261..309 /label=HSV TK poly(A) signal /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" protein_bind complement(511..544) /label=Cre recombinase binding site /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin 546..902 /label=R6K gamma ori /note="R6K gamma ori" /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS 1365..2156 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=aadA /note="SmR" /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa 61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta 121 caatcactcg acgaagactt gatcacccgg gatctcgagc catggtgcta gcagctgatg 181 catagcatgc ggtaccggga gatgggggag gctaactgaa acacggaagg agacaatacc 241 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt 301 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 361 gaccccattg ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt 421 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacta 481 cgggtacgtt taaacccatg tgcctggcag ataacttcgt ataatgtatg ctatacgaag 541 ttatggtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa 601 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg 661 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa 721 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa 781 ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact 841 gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga 901 cataattcag cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg 961 aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctgggag 1021 gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg 1081 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaagt 1141 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 1201 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg 1261 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg 1321 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga 1381 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac 1441 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca 1501 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt 1561 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg 1621 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg 1681 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca 1741 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg 1801 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc 1861 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg 1921 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg 1981 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg 2041 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt 2101 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc 2161 taacaattcg ttcaagccga cggatctatg tcgggtgcgg agaaagaggt aatgaaatgg 2221 cacctaggta t //