LOCUS       Exported                4944 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(275..1090)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1212..1800
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  2105..2329
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2396..2929)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=aacC1
                     /note="GmR"
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3118..3146)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=intI1 (promoter lies within the coding sequence
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     protein_bind    3542..3575
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3765..3813
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(3949..4058)
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        4103..4194
                     /gene="polh from Autographa californica"
                     /label=polh from Autographa californica promoter
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4453..4587
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     mobile_element  4697..4862
                     /mobile_element_type="transposon:Tn7"
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgcca aacgtactgc
      241 ggtaaagctc atcagcgtgg tcgtgaagca attcttagaa aaactcatcg agcatcaaat
      301 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
      361 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
      421 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
      481 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
      541 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
      601 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
      661 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
      721 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
      781 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
      841 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
      901 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
      961 acaagcgata gattgtcgca cctgattgcc cgacattatc tcgagcccat ttatacccat
     1021 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     1081 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     1141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1321 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1981 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac
     2041 cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag
     2101 cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata
     2161 aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa
     2221 gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc
     2281 cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg
     2341 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     2401 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     2461 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     2521 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     2581 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     2641 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     2701 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     2761 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     2821 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     2881 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     2941 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     3001 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     3061 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     3121 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     3181 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac
     3241 gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac
     3301 ggcaaggtgc tgtgcacgga tctgcccttg cttcaggaga tcggtagacc tcggccgtcg
     3361 cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa
     3421 ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacagctt
     3481 tgtttgtact atcaacaggt tgaactgctg atcaacagat cctctacgcg gccgcggtac
     3541 cataacttcg tatagcatac attatacgaa gttatctggt ttaaacgtac ccgtagtggc
     3601 tatggcaggg cttgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg
     3661 gggttggggt ggggaaaagg aagaaacgcg ggcgtattgg tcccaatggg gtctcggtgg
     3721 ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac
     3781 acccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     3841 tctccttccg tgtttcagtt agcctccccc atctcccggt accgcatgct atgcatcagc
     3901 tgctagcacc atggctcgag atcccgggtg atcaagtctt cgtcgagtga ttgtaaataa
     3961 aatgtaattt acagtatagt attttaatta atatacaaat gatttgataa taattcttat
     4021 ttaactataa tatattgtgt tgggttgaat taaaggtccg tatactagta tcgattcgcg
     4081 acctactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     4141 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     4201 gattattcat accgtcccac catcgggcgc ggatcccggt ccgaagcgcg cggaattcaa
     4261 aggcctacgt cgacgagctc actagtcgcg gccgctttcg aatctagagc ctgcagtctc
     4321 gacaagcttg tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt
     4381 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     4441 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     4501 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     4561 atcaatgtat cttatcatgt ctggatctga tcactgcttg agcctagaag atccggctgc
     4621 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactatcata
     4681 acccctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     4741 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     4801 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     4861 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     4921 caaaccgtca tcttcggcta cttt
//
