LOCUS       Exported                2855 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(20..53)
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      106..462
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(857..1516)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1517..1619)
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     polyA_signal    1791..1925
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2167..2185)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2230..2433)
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2434..2813
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aaacccatgt gcctggcaga taacttcgta taatgtatgc tatacgaagt tatggtaccg
       61 cggccgcgta gaggatctgt tgatcagcag ttcaacctgt tgatagtacg tactaagctc
      121 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
      181 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
      241 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
      301 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
      361 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
      421 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt agcttcacgc
      481 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
      541 ccgcagaaac ggtgctgacc ccggatgaat gtcagctggg aggcagaata aatgatcata
      601 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
      661 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      721 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
      781 cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat
      841 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      901 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      961 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1021 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1081 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1141 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1201 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1261 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     1321 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1381 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1441 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1501 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1561 cgcccggtag tgatcttatt tcattatggt gaaagttgga ccctcttacg tgccgatcaa
     1621 cgtctcattt tcgccaaaag ttggcccaga tctatgtcgg gtgcggagaa agaggtaatg
     1681 aaatggcacc taggggttat gatagttatt gctcagcggt ggcagcagcc aactcagctt
     1741 cctttcgggc tttgttagca gccggatctt ctaggctcaa gcagtgatca gatccagaca
     1801 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1861 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1921 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1981 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagtac
     2041 ttctcgacaa gcttgtcgag actgcaggct ctagattcga aagcggccgc gactagtgag
     2101 ctcgtcgacg taggcctttg aattccgcgc gcttcggacc gggatccact agccagcttg
     2161 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
     2221 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     2281 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     2341 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     2401 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     2461 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     2521 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     2581 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     2641 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     2701 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     2761 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     2821 atatctggcc cgtacatcgc gaatcgatac tagta
//
