LOCUS       Exported                4130 bp ds-DNA     circular SYN 07-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 7, 2017 from SnapGene Viewer 3.2.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             90..884
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        1488..1763
                     /note="chicken beta-actin promoter"
     intron          1764..2773
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     polyA_site      3058..3113
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     protein_bind    complement(3468..3501)
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      3503..3859
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg
       61 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
      121 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
      181 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
      241 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
      301 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
      361 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
      421 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
      481 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
      541 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
      601 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgaca catggcgatg
      661 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
      721 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
      781 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
      841 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
      901 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
      961 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     1021 gcgcggggat ctcatgctgg agttcttcgc ccaccccggg atctatgtcg ggtgcggaga
     1081 aagaggtaat gaaatggcac ctaggtatcg atactagtat acgttattaa tagtaatcaa
     1141 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     1201 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     1261 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt
     1321 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1381 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1441 ctacttggca gtacatctac gtattagtca tcgctattac tcatgggtcg aggtgagccc
     1501 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1561 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc gcgccaggcg
     1621 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
     1681 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     1741 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc
     1801 gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     1861 ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt
     1921 cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg
     1981 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc
     2041 tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg
     2101 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa
     2161 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg
     2221 ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     2281 ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     2341 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
     2401 cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     2461 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg
     2521 ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa
     2581 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca
     2641 gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     2701 ggcttctggc gtgtgaccgg cggctgctag agcctctgct aaccatgttc atgccttctt
     2761 ctttttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
     2821 agaattgcgg ccgtctcagg ccaccgaaga cttgatcacc cgggatctcg agccatggtg
     2881 ctagcagctg atgcatagca tgcggtacct aattcactcc tcaggtgcag gctgcctatc
     2941 agaaggtggt ggctggtgtg gctaatgccc tggctcacaa ataccactga gatctttttc
     3001 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     3061 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     3121 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     3181 caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg tcatcagtat
     3241 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     3301 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     3361 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     3421 cttctcttat gaagatccct cgacgtttaa acccatgtgc ctggcagata acttcgtata
     3481 atgtatgcta tacgaagtta tggtacgtac taagctctca tgtttcacgt actaagctct
     3541 catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta
     3601 agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa
     3661 caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga
     3721 aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg gacaacaagc
     3781 cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttc agtgacacag
     3841 gaacacttaa cggctgacag aattagcttc acgctgccgc aagcactcag ggcgcaaggg
     3901 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     3961 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
     4021 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
     4081 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
//
