LOCUS       Exported                4088 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        388..663
                     /note="chicken beta-actin promoter"
     intron          664..1673
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     polyA_site      1958..2013
                     /label=beta-globin poly(A) signal
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     protein_bind    complement(2368..2401)
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      2403..2759
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             3222..4013
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=aadA
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactacg atactagtat acgttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac tcatgggtcg aggtgagccc cacgttctgc ttcactctcc
      421 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
      481 cagcgatggg ggcggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa
      841 agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc tgccgcaggg
     1561 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1621 cggctgctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattgcgg ccgtctcagg
     1741 ccaccgaaga cttgatcacc cgggatctcg agccatggtg ctagcagctg atgcatagca
     1801 tgcggtacct aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     1861 gctaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     1921 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     1981 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     2041 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg
     2101 ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     2161 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     2221 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     2281 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     2341 cgacgtttaa acccatgtgc ctggcagata acttcgtata atgtatgcta tacgaagtta
     2401 tggtacgtac taagctctca tgtttcacgt actaagctct catgtttaac gtactaagct
     2461 ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg gctaacgtac
     2521 taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt aatataaatc
     2581 agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa tatataaggc
     2641 ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatgta acgcactgag
     2701 aagcccttag agcctctcaa agcaattttg agtgacacag gaacacttaa cggctgacat
     2761 aattcagctt cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac
     2821 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctgggaggca
     2881 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     2941 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaagtagc
     3001 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     3061 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     3121 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     3181 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     3241 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     3301 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     3361 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     3421 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     3481 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     3541 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     3601 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3661 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3721 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3781 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3841 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3901 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3961 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa
     4021 caattcgttc aagccgacgg atctatgtcg ggtgcggaga aagaggtaat gaaatggcac
     4081 ctaggtat
//
