LOCUS       Exported                2858 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      7..363
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    416..449
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    639..687
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(823..932)
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        977..1068
                     /gene="polh from Autographa californica"
                     /label=polh from Autographa californica promoter
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1327..1461
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1611..2402)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=aadA
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaattctgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
       61 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      121 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      181 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
      241 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
      301 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
      361 tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg gtaccataac
      421 ttcgtatagc atacattata cgaagttatc tggtttaaac gtacccgtag tggctatggc
      481 agggcttgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttgggggttg
      541 gggtggggaa aaggaagaaa cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc
      601 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt
      661 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
      721 tccgtgtttc agttagcctc ccccatctcc cggtaccgca tgctatgcat cagctgctag
      781 caccatggct cgagatcccg ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta
      841 atttacagta tagtatttta attaatatac aaatgatttg ataataattc ttatttaact
      901 ataatatatt gtgttgggtt gaattaaagg tccgtatact agtatcgatt cgcgacctac
      961 tccggaatat taatagatca tggagataat taaaatgata accatctcgc aaataaataa
     1021 gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat tccggattat
     1081 tcataccgtc ccaccatcgg gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct
     1141 acgtcgacga gctcactagt cgcggccgct ttcgaatcta gagcctgcag tctcgacaag
     1201 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     1261 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1321 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1381 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1441 gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa
     1501 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat cataacccct
     1561 aggagtccaa gcgagctcga tatccgtcgg cttgaacgaa ttgttagaca ttatttgccg
     1621 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
     1681 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
     1741 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
     1801 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
     1861 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
     1921 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
     1981 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
     2041 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
     2101 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
     2161 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
     2221 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
     2281 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
     2341 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
     2401 atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
     2461 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2521 tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2581 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt accggtttat
     2641 tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg
     2701 ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc cagctgacat
     2761 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     2821 agcccttgcg ccctgagtgc ttgcggcagc gtgaagct
//
