LOCUS       Exported                3005 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 19, 2019 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      6..360
                     /label=R6K gamma ori
                     /note="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    413..446
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    636..684
                     /label=HSV TK poly(A) signal
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(820..929)
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        974..1065
                     /gene="polh from Autographa californica"
                     /label=polh from Autographa californica promoter
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    1324..1458
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        1855..1957
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1958..2617
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aattctgtca gccgttaagt gttcctgtgt cactgaaaat tgctttgaga ggctctaagg
       61 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt
      121 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
      181 tgctgattta tattaatttt atggtcaaac agagagctta gtacgtgaaa catgagagct
      241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
      301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
      361 tatcaacagg ttgaactgct gatcaacaga tcctctacgc ggccgcggta ccataacttc
      421 gtatagcata cattatacga agttatctgg tttaaacgta cccgtagtgg ctatggcagg
      481 gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg
      541 tggggaaaag gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac
      601 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg
      661 ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
      721 gtgtttcagt tagcctcccc catctcccgg taccgcatgc tatgcatcag ctgctagcac
      781 catggctcga gatcccgggt gatcaagtct tcgtcgagtg attgtaaata aaatgtaatt
      841 tacagtatag tattttaatt aatatacaaa tgatttgata ataattctta tttaactata
      901 atatattgtg ttgggttgaa ttaaaggtcc gtatactagt atcgattcgc gacctactcc
      961 ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta
     1021 ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca
     1081 taccgtccca ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg
     1141 tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct cgacaagctt
     1201 gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
     1261 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1321 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1441 tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg ctaacaaagc
     1501 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     1561 ggcctctaaa cgggtcaacc cctaggggcc tctaaacggg tcttgagggg ttttttgctg
     1621 aaaggaggaa ctatatccgg atctgaacag gagggacagc tgatagaaac agaagccact
     1681 ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccgacgc
     1741 actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg
     1801 tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg
     1861 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1921 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1981 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2041 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     2101 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     2161 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     2221 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     2281 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     2341 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     2401 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     2461 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     2521 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     2581 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     2641 cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     2701 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
     2761 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
     2821 atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca
     2881 tttattctgc ctcccagagc ctgacattca tccggggtca gcaccgtttc tgcggactgg
     2941 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg
     3001 aagct
//
