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BacMam Compatible Cells

Compatibility of Mammalian Cells for Transduction

Human cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • 143B (osteosarcoma, ATCC CRL-8303)
  • 39
  • >90%
  • 143TK- (fibroblast)
  • 10
  • N/A
  • A549 (ATCC CCL-185, lung carcinoma)
  • 41
  • N/A
  • BGC-823 (gastric carcinoma)
  • 39
  • 80%
  • Bone marrow fibroblasts
  • 5
  • N/A
  • C3A liver cells
  • 29
  • N/A
  • Cal-51 (human mammary carcinoma)
  • M. Mueller, U. Heidelberg
  • 50% – 60%
  • CHP212 (neuroblastoma)
  • 9
  • N/A
  • Colo-205 epithelial cells
  • C. Henery, Amnis Corp.
  • 70% – 80%
  • CRL-1973 (NTERA-2, Nt-2; embryonal carcinoma)
  • 4, Molecular Probes
  • N/A
  • DLS-1
  • 13
  • N/A
  • DMS 114 (ATCC CRL-2066, small cell lung carcinoma)
  • 39
  • 80%
  • EA.hy926 (hybridoma of HUVEC and A549)
  • 41
  • 10% – 70%
  • Embryonic lung fibroblasts
  • 11
  • N/A
  • FLC4 (human hepatocarcinoma)
  • 15
  • N/A
  • Glioma
  • Genetech, 28, 35, 46, 50
  • 80% – 90%
  • HEK 293
  • 2,5,15,27,39
  • >90%
  • HeLa
  • 4,5,9,15,18,19,39
  • 60% – >90%
  • HepG2 (ATCC HB-8065, hepatocellular carcinoma)
  • 1,2,15,34, 39, 47
  • >90%
  • HuH-7 (hepatoma)
  • 1,4,5,15,19, 53
  • >90%
  • Human adipose mesenchymal stem cells (MSC)
  • Geneva Biotech
  • >90%
  • Human bone-marrow mesenchymal stem cells (MSC)
  • Geneva Biotech
  • 80%
  • Human dendtric cells
  • 25
  • 15%
  • Human embryonic stem cells (HES)
  • Geneva Biotech, 30
  • 25% – 80%
  • Human embryonic neural stem cells
  • 9
  • 30%
  • Human inducible pluripotent stem cells (iPSC)
  • 54
  • >70
  • Human mesenchymal stem cells (MSC)
  • 20, 49
  • 70%
  • IMR-32 neuroblastoma (ATCC CCL-127)
  • 4,32
  • N/A
  • K-562 (ATCC CCL-243, chronic myelogenous leukemia)
  • Geneva Biotech
  • >80%
  • KATO-III (HTB-103, gastric carcinoma)
  • 4
  • N/A
  • Keratinocytes
  • 5
  • N/A
  • LNCaP (human prostatic adenocarcinoma)
  • F.Matthieu, U. Science et technologies de Lille
  • N/A
  • MCF7 (ATCC HTB-22D, breast cancer cell line)
  • NIH-NCI, 39
  • >80%
  • MG63 (ATCC CRL-1427, osteosarcoma)
  • 5
  • >90%
  • MRC-5 (lung fibroblast)
  • 7, 53
  • N/A
  • Pancreatic b-cells
  • 8
  • N/A
  • PLC/PRF/5 (hepatoma; ATCC CRL-8024)
  • 39
  • >90%
  • Prenatal cardiomyocytes (hCM)
  • 18
  • N/A
  • Primary bone marrow fibroblasts
  • 5
  • 80%
  • Primary dendritic cells
  • 25
  • N/A
  • Primary human aortic smooth muscle cells (HASMC)
  • Cascade Biologics
  • >80%
  • Primary human astrocytes (T98G and U373-MG)
  • 9, 58
  • >50%
  • Primary human cardiomyocytes
  • 18
  • 90%
  • Primary human chondrocytes
  • NIH
  • N/A
  • Primary human coronary artery endothelia cells (HCEC)
  • 18
  • 40%
  • Primary human coronary smooth muscle cells (HCASMC)
  • 18
  • 80%
  • Primary human dermal fibroblasts – adult (HDFa)
  • Cascade Biologics
  • >90%
  • Primary human dermal fibroblasts – neonatal (HDFn)
  • Cascade Biologics
  • >90%
  • Primary human fibroblasts (HFB)
  • 18, Cascade Biologics
  • >90%
  • Primary human foreskin fibroblasts (HFF)
  • 5, 12, 28
  • 30%
  • Primary human glial cells
  • 9
  • ~60%
  • Primary human hepatic stellate cells
  • 19, 23
  • 90%
  • Primary human hepatocytes
  • 1, 2, 5, Molecular Probes
  • >90%
  • Primary human keratinocytes (HEK)
  • 5, Cascade Biologics
  • >90%
  • Primary human lung fibroblasts
  • NIH
  • N/A
  • Primary human mammary epithelial cells (HMEC)
  • Cascade Biologics
  • >90%
  • Primary human melanocytes
  • Geneva Biotech
  • >90%
  • Primary human neuoepithelial and neuroblastic cells
  • 9
  • N/A
  • Primary human pancreatic islet cells
  • 8
  • N/A
  • Primary umbilical vein endothelial cells (HUVEC)
  • 54, 28, Geneva Biotech
  • 90%
  • Saos-2
  • 5,6,19, 38
  • >90%
  • SHSY-5Y (neuroblastoma)
  • 27,29
  • N/A
  • SK-BR-3
  • Molecular Probes
  • N/A
  • SK-N-MC
  • 5
  • >90%
  • SK-OV-3 (ATCC HTB-77, adenocarcinoma)
  • 50
  • N/A
  • T47D (breast carcinoma)
  • 36
  • N/A
  • U-2 OS
  • 27, Molecular Probes
  • >90%
  • W12 (human cervical keratinocyte)
  • 5
  • >90%
  • WI38 (human lung fibroblast)
  • 5, 39
  • >90%

Non-human primate cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • COS-7 (African green monkey kidney fibroblast-like cells)
  • 3,4,5,9, Molecular Probes
  • >90%
  • CV-1 (African green monkey kidney fibroblast cells)
  • 5,15, 39, Molecular Probes
  • >90%
  • Vero
  • 14, A.Snyder, OHSU
  • 50%

Bovine cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • MDB (bovine kidney) epithelial cell line
  • 5
  • N/A
  • BT (bovine turbinate) epithelial cell line (ATCC CRL-1390)
  • 5
  • N/A

Ovine (Sheep) cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • FLL-YFT (fetal lamb lung) cell line
  • 5
  • N/A

Cervidae (Deer) cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • Indian Muntjac deer epidermis cell line
  • M.Davidson, Molecular Expressions Inc.
  • N/A

Suis (Pig) cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • CPK (porcine kindney)
  • 4,5
  • N/A
  • FS-L3 (porcine kidney) epithelial cell line
  • 4,5
  • N/A
  • PK-15 (porcine kidney; ATCC CCL-33)
  • 5,34
  • N/A
  • Left atrial appendage progenitor cells – adult stem cells
  • M.Rutten and K.Gregory, OMLC
  • N/A
  • Porcine coronary artery smooth muscle cells (pCSMC)
  • 18
  • LLC-PK1 (kidney proximal tubule) cell line
  • 27, 56
  • >50%
  • Primary Cardiac Smooth Muscle Cells
  • M.Rutten and K.Gregory, OMLC
  • N/A

Carnivores

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • FoLu (Gray fox lung fibroblasts)
  • M. Davidson, Molecular Expressions Inc.
  • N/A
  • MDCK (NBL-2; dog kidney)
  • Molecular Probes
  • N/A

Marsupials

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • OK (opossum kidney) epithelial cell line
  • M.Davidson, Molecular Expressions Inc.
  • N/A

Hamster cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • CHO (Chinese hamster ovary K1, M1WT3, hIR)
  • 5,15,31, Molecular Probes
  • 75% – >90%

Mouse cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • 3T3 mouse fibroblasts
  • 23, 38, 39, Geneva Biotech
  • 15% – 80%
  • BNL 1ME A7.7R.1 (ATCC TIB-75, mouse liver carcinoma)
  • 39
  • 75%
  • C2C12 (myoblast)
  • 51
  • 60%
  • Dendritic cells
  • 36
  • 15%
  • GnRH neuronal cells
  • S.Singh, Johns Hopkins
  • 60% – 70%
  • JC (adenocarcinoma)
  • 39
  • 20%
  • L929 (ATCC CCL-1, subcutaneous connective tissue)
  • 39
  • 35%
  • MEF
  • 54,57, 59
  • >70%
  • Neuroblastoma (N2a)
  • 9
  • N/A
  • P388D1 (ATCC CCL-46, lymphoma)
  • 5
  • 25%
  • Primary kidney cells
  • 22
  • N/A
  • Primary pancreatic acinar cell
  • Geneva Biotech
  • N/A
  • Primary pancreatic islet cells
  • 8
  • 85%
  • Primary retinal cells
  • 55
  • NA
  • Primary ventricular cardiomyocytes
  • Geneva Biotech
  • >90%
  • PT67 (embryo fibroblast)
  • 39
  • 10%
  • Sol 8 (myoblast)
  • 51
  • 75%

Potoroo (Rat Kangaroo) cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • Ptk2
  • M.Davidson, Molecular Expressions Inc.
  • N/A

Rabbit cells

  • CELL TYPE
  • REFERENCE (see below)
  • TRANSDUCTION %
  • CRL-2560 (RH/K30, MT-2; rabbit T-cell line)
  • 4
  • N/A
  • Primary aortic smooth muscle cells (RaASMC)
  • 41
  • 40% – 75%
  • Primary chondrocytes
  • 44
  • >75%
  • Primary chondrocytes (intervertebral disc nucleus)
  • 24
  • 85%
  • Primary hepatocytes
  • 16
  • N/A
  • RK13 (normal rabbit kidney epithelial cells)
  • C. Harrison, Univ. Melbourne
  • >80%

Rat cells

  • BHK
  • 5,15,34
  • N/A
  • Brain choroid plexus cells (in vivo)
  • 40
  • 60% – >90%
  • Brain pericytes cell line
  • Molecular Probes
  • N/A
  • C17.2 cells (multipotent neural stem cell line)
  • 31
  • N/A
  • Neural stem cells
  • A.Moutri, Salk Institute
  • 30%
  • PC12
  • 4,5
  • 20%
  • Primary cerebellar granule neurons
  • 31
  • N/A
  • Primary chondrocytes
  • 21, 42, 45
  • 85%
  • Primary cortical neurons
  • 54, Geneva Biotech
  • >90%
  • Primary dissociated dorsal root ganglion neurons
  • 55
  • >80%
  • Primary glial cells (astrocytes)
  • 48
  • 60% – 80%
  • Primary hepatic stellate cells
  • 23
  • Primary hepatocytes
  • 2
  • N/A
  • Primary myoblasts
  • 51
  • 75%
  • Primary rat tendon fibroblasts
  • K. Gardner, Michigan State Univ.
  • N/A
  • Primary spiral ganglion neurons
  • 33
  • N/A
  • Rat2
  • M.Davidson, Molecular Expressions Inc.
  • REF(rat embryo fibroblast)
  • 54
  • >70%
  • RGM I
  • 4
  • N/A
  • T6 (rat hepatic stellate cell line)
  • 23
  • 20%

Zebrafish

  • Zebrafish
  • 54,55
  • N/A

References forBacMam-mediated Transduction of Mammalian Cells

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  • 2. Boyce, F.M. & Boucher, N. (1996) Proc. Natl. Acad. Sci. USA 93:2348-2352
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  • 3. Yap, C.-C. et al. (1997) Virology 231:192-200
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  • 4. Shoji, I. et al. (1997) J. Gen. Virol. 78:2657-2664
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  • 6. Merrihew, R.V. et al. (2001) J. Virol. 75:903-909
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  • 8. Ma, L. et al. (2000) Diabetes 49:1986-1991
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