We design viral vector systems that can deliver complex, multigene cargos into diverse cell types: insect cells, mammalian cells, primary cells and whole organs, for a variety of applications. We have expertise in viral vector genome engineering including development of pseudotyping strategies, target homing and cell entry strategies for the most difficult cell and organ types. Our goal in these applications is the same: to develop methods to deliver DNA code of ever increasing size and complexity into cells.
The first method for delivering a multigene readout into diverse mammalian and primary cells lines is described in Multigene Delivery in Primary Cells_Nature Communications_2016.
We follow a well estabilished strategy of synthetic biology and carry out iterative cycles of i) design and engineering ii) DNA assembly, delivery into cells & production and iii) testing to produce a toolbox of synthetic biobricks, circuits and chassis