Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech MultiColi™

Intelligent DNA delivery systems

Welcome Guest \\ Login


Technology Description

While multi-protein expression remains a formidable task and generating sufficiently soluble protein complexes at times requires some tweaking, such challenges now become easier with MultiColi™ a versatile and convenient Acceptor-Donor vector system for manual or automated recombineering of genes destined for expression in E. coli. The MultiColi™ technology is the first fully automatable system for protein complex production in E.coli. Originally developed as part of EMBL’s ACEMBL pipeline, which is a centerpiece of the European Commission Framework Programme 7 project ComplexINC, MultiColi™ sets new standards for multi-protein expression in this versatile laboratory host organism.

MultiColi™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.

Schematic Diagram of MultiColi Heterologous Protein Expression System

How MultiColi™ Works:

  • Left) Design and clone your gene(s) of interest (GOIs) into MultiColi™ acceptor and donor vectors
  • Center) Match and mix your GOIs and then (re)combine them into one construct via Cre-Lox recombination, and select your construct using unique combination of antibiotic markers
  • Right) Transform E. coli cells with composite GOI vector. Expression follows via induction.
    Resulting multiprotein expression product can be used for biochemical analysis, structural biology, drug discovery etc.

References and Downloads:

Click here to download Bieniossek et al 2009. Nature Methods.

Click here to download Nie et al 2009. Nature Protocols.

Click here to download MultiColi™ User Manual v5.1

Click here for DNA vector sequences

Click here to download Cre-ACEMBLER User Manual 3.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows

Click here to download Cre-ACEMBLER for Linux


MultiColi™ Kit + Competent Cells 1,490.00€ ENQUIRY

Chemically Competent Cells

PirHC (12 x 100µl) 960.00€ ENQUIRY
ig™ 10B (12 X 100µl) 760.00€ ENQUIRY


pACE1 (5µg) 279.00€ ENQUIRY
pACE2 (5µg) 279.00€ ENQUIRY
pDC (5µg) 279.00€ ENQUIRY
pDK (5µg) 279.00€ ENQUIRY
pDS (5µg) 279.00€ ENQUIRY


E.coli cell expression

Research Highlights

our r&d View all

Latest News

DISTRIBUTOR IN CHINA Geneva Biotech is delighted to announce better services for our customers in China by teaming up with Equl LTD who have ...

View all

Contact Us