The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. MultiLabel™ is a novel and highly efficient modular plasmid-based eukaryotic expression system.
MultiLabel™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.
How MultiLabel™ Works:
- 1) Design and clone your gene(s) of interest (GOIs) into MultiMam™ Transient acceptor and donor vectors
- 2) Match and mix your GOIs and then (re)combine them into one construct
- 3) Select your construct using unique combination of antibiotic markers
- 4) Transfect mammalian cells with composite GOI vector. Transient expression follows.
- 5) Drug discovery, bioproduction, stem cell differentiation etc.
References and Downloads:
Mansouri et al. 2016. Nature Communications..
Click here to download Kriz et al 2010
Click here to download Cre-ACEMBLER User Manual v2.0
Click here to download Cre-ACEMBLER Short Description
Click here to download Cre-ACEMBLER for Windows (32bit)
Click here to download Cre-ACEMBLER for Linux