Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech MultiPrime™

Intelligent DNA delivery systems

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MultiPrime™

Technology Description

BacMam is an increasingly popular system that facilitates delivery of heterologous DNAs to broad range of mammalian transduction targets. BacMam uses baculovirus as a vector, and has distinct advantages over other mammalian transfection methods given the large heterologous DNA carrying capacity of baculovirus (>40kbp), the uptake of baculovirus by a broad range of mammalian cell types, and the sophisticated molecular biology technologies that have evolved over the last 20 years to support generation of baculovirus expression vectors.

MultiPrime™ is the first BacMam system designed specifically to allow modular, DNA recombination based assembly of multiexpression cassettes for multiprotein co-expression in mammalian cells. MultiPrime™ uses the same DNA recombination technologies as MultiBac™, but with mammalian promoters, and a "mammalianized" baculovirus- displaying a VSV peptide on the baculovirus surface that increases virus uptake by an order of magnitude, and a stably integrated fluorescent protein to simplify monitoring of experiments.

MultiPrime™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The MultiBacMam™ baculovirus genome (DH10EMBacVSV) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots, and contains an integrated fluorescent protein expression cassette to allow for ease of monitoring virus titer. The MultiBacMam™ virus particle displays the VSV peptide on its surface to increase efficiency of entry into mammalian cells by around 1 order of magnitude compared to prior BacMam virus systems.

MultiPrime™ allows you to run the assays you need in the cellular models you want. MultiBacMam™ is first gene delivery system that easily allows for multiple factors to be delivered to the target. The first to allow expression of multi-component pathways (e.g. signal cascades) for drug discovery in mammalian cells, without the need for complicated multi vector co-transfections.

 

How MultiPrime™ Works:

MultiBacMam
  1. Design and clone your gene(s) of interest (GOIs) into MultiBacMam™ acceptor and donor vectors
  2. Match and mix your GOIs and then (re)combine them into one construct
  3. Select your construct using unique combination of antibiotic markers
  4. Transfer the entire GOIs-assembly into the MultiBacMam™ bacmid
  5. Select and amplify your gene-containing MultiBacMam™ bacmid
  6. Transfect insect cells with purified MultiBacMam™ bacmid
  7. Amplify baculovirus in insect cells and collect desired scale of virus
  8. Transfect mammalian cells with baculovirus
  9. Drug discovery, bioproduction, stem cell differentiation etc.
Baculovirus Based Gene Delivery Using MultiBacMam

MultiPrime™. Recombinant baculoviruses are a useful tool for multi-gene transfer in mammalian cells.

Click A Reference to Download:

Mansouri et al. 2016. Nature Communications..

Trowitzsch et al. 2011. Bioessays 33, 946–955.

Click Here to Download MultiBacMam™ User Manual v1.2

Click here to download Cre-ACEMBLER User Manual v2.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows (32bit)

Click here to download Cre-ACEMBLER for Linux

Kits

MultiPrime™ Expression System Kit 1490.00€ ENQUIRY

Competent Cells

DH10EMBacVSV (12 x 100µl) 960.00€ ENQUIRY
PirHC (12 x 100µl) 960.00€ ENQUIRY
ig™ 10B (12 X 100µl) 760.00€ ENQUIRY

Plasmids

pACEMam1 (5µg) 279.00€ ENQUIRY
pACEMam2 (5µg) 279.00€ ENQUIRY
pMDC (5µg) 279.00€ ENQUIRY
pMDK (5µg) 279.00€ ENQUIRY
pMDS (5µg) 279.00€ ENQUIRY

Dual Plasmids

pUCDM MultiBacMam Cargo (5µg) 419.00€ ENQUIRY
pUCDM MultiBacMam Cargo_GFP (5µg) 419.00€ ENQUIRY

Dual Mammalian Insect Cell Plasmids

pFL-IM (5µg) 419.00€ ENQUIRY
pKL-IM (5µg) 419.00€ ENQUIRY
pSPL-IM (5µg) 419.00€ ENQUIRY
pUCDM-IM (5µg) 419.00€ ENQUIRY

Enquiry

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