The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. MultiMam™ is a novel and highly efficient modular plasmid-based eukaryotic expression system.
A specific subset of transfer vectors called “MutiLabel” were developed by Kriz et al (see reference download link below) and allow scientists to precisely label many cellular components involved in a given disease process and follow them all at the same time. A demonstration of these vectors in action is shown here Link
MultiMam™ Transient and MultiLabel transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.