Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech MultBacTAG™

Intelligent DNA delivery systems

Welcome Guest \\ Login


Technology Description


Genetic code expansion (GCE) is a powerful method to incorporate artificial amino acids into polypeptide chains to create synthetic proteins with novel functions, with many applications ranging from discovery science to molecular medicine. Until recently, this method has been mostly confined to small individual proteins representing a limited repertoire of cellular activity. Biological function in humans, however, is typically catalyzed by large protein machines, often comprising ten or more individual protein subunits. An international team of scientists from University of Bristol, EMBL and the Karlsruhe Institute of Technology developed MultiBacTAG™, a powerful system to enable genetic code expansion in complex multiprotein machines.

Scientists can now conveniently produce recombinant eukaryotic proteins with synthetic amino-acids inserted at specific locations, thanks to the MultiBacTAG™. MultiBacTAG’s applications include glycoengineering proteins compatible with human tissue studies, fluorescence labelling of specific targets to measure structure and dynamics in proteins, and amino acid cross-linking in order to map protein binding interfaces.

The platform can provide insight into how specific protein complexes function, as well as possibilities to custom-design proteins for therapeutic biotechnology and pharmaceutical applications. In their paper, for example, the team used MultiBacTAG to engineer Herceptin – an antibody that associates with cancer cells – to recognize breast cancer cells in human tissue.


How MultiBac™ Works:

  • 1) Design and clone gene(s) of interest (GOIs) into MultiBac™ acceptor and donor vectors
  • 2) Match and mix your GOIs and then (re)combine them into one construct
  • 3) Select your construct using unique combination of antibiotic resistance markers
  • 4) Transfer the entire GOIs-assembly into the MultiBac™ bacmid
  • 5) Select and amplify your gene-containing MultiBac™ bacmid
  • 6) Transfect insect cells with purified MultiBac™ bacmid
  • 7) Expression of protein(s) in insect cells

Click a Reference to Download:

User Manual and Other Downloads

Click here to download MultiBac™ User Manual v8.5

Click here for DNA vector sequences

Click here to download MultiBac™ Short Description

Click here to download Cre-ACEMBLER User Manual 3.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows

Click here to download Cre-ACEMBLER for Linux


MultiBacTAG™ Kit + Competent Cells 1,490.00€ ENQUIRY

Chemically Competent Cells

DH10EMBacY (12 x 100µl) 960.00€ ENQUIRY
DH10MultiBac (12 x 100µl) 960.00€ ENQUIRY
ig™ 10B (12 X 100µl) 760.00€ ENQUIRY
PirHC (12 x 100µl) 960.00€ ENQUIRY


pACEBac1 (5µg) 279.00€ ENQUIRY
pACEBac2 (5µg) 279.00€ ENQUIRY
pIDK (5µg) 279.00€ ENQUIRY
pIDC (5µg) 279.00€ ENQUIRY
pIDS (5µg) 279.00€ ENQUIRY

Dual Plasmids

pFL (5µg) 319.00€ ENQUIRY
pKL (5µg) 319.00€ ENQUIRY
pSPL (5µg) 319.00€ ENQUIRY
pUCDM (5µg) 319.00€ ENQUIRY

Dual Mammalian Insect Cell Plasmids

pFL-IM (5µg) 419.00€ ENQUIRY
pKL-IM (5µg) 419.00€ ENQUIRY
pSPL-IM (5µg) 419.00€ ENQUIRY
pUCDM-IM (5µg) 419.00€ ENQUIRY


E.coli cell expression

Research Highlights

our r&d View all

Contact Us