ComplexLink™ is a system of polyprotein production combined with co-production of sequence-specific proteases that co-translationally cleave the polyprotein into functional monomeric subunits. The technology provides unique advantages over existing expression systems for production of multiprotein complexes where subunit stoichiometry is difficult to control. The technology was applied to produce recombinant influenza polymerase complex from a self-processing polyprotein, as recently published in Nature (see links below).
The influenza polymerase remained inaccessible for decades – the challenge to produce this highly complicated protein complex for detailed analysis, proved to be a seemingly insurmountable technical challenge. The break-through studies, from the European Molecular Biology Labs, used Geneva Biotech co-founder Imre Berger’s ComplexLINK technology to produce recombinant influenza polymerase complex from a self-processing polyprotein, as recently published in Nature (links below).