Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

Geneva Biotech MultiBacMam™

Intelligent DNA delivery systems

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MultiBacMam™

Technology Description

BacMam is an increasingly popular system that facilitates delivery of heterologous DNAs to broad range of mammalian transduction targets. BacMam uses baculovirus as a vector, and has distinct advantages over other mammalian transfection methods given the large heterologous DNA carrying capacity of baculovirus (>40kbp), the uptake of baculovirus by a broad range of mammalian cell types, and the sophisticated molecular biology technologies that have evolved over the last 20 years to support generation of baculovirus expression vectors.

MultiBacMam™ is the first BacMam system designed specifically to allow modular, DNA recombination based assembly of multiexpression cassettes for multiprotein co-expression in mammalian cells. MultiBacMam™ uses the same DNA recombination technologies as MultiBac™, but with mammalian promoters, and a "mammalianized" baculovirus- displaying a VSV peptide on the baculovirus surface that increases virus uptake by an order of magnitude, and a stably integrated fluorescent protein to simplify monitoring of experiments.

MultiBacMam™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The MultiBacMam™ baculovirus genome (DH10EMBacVSV) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots, and contains an integrated fluorescent protein expression cassette to allow for ease of monitoring virus titer. The MultiBacMam™ virus particle displays the VSV peptide on its surface to increase efficiency of entry into mammalian cells by around 1 order of magnitude compared to prior BacMam virus systems.

A specific subset of transfer vectors for use in primary cells called “MutiPrime” were developed by Mansouri et al 2016 (Nature Communications 2016, see reference download link below).

To advance the MultiPrime technology, Geneva Biotech and collaborators are developing a series of additional primary cell-specific transduction systems which specialize in tough to transduce cell types.

MultiBacMam™ vectors together with the MultiPrime vectors allows you to run the assays you need in the cellular models you want. This technology is first gene delivery system that easily allows for multiple DNA-encoded factors to be delivered on one vector into mammalian cells, tissues, or organisms. It is the first to allow expression of multi-component pathways (e.g. signal cascades), or complex targets such as multicomponent ion channels for drug discovery in mammalian cells tissues or organisms, all without the need for complicated multi vector co-transfections.

 

How MultiBacMam™ Works:

MultiBacMam
  1. Design and clone your gene(s) of interest (GOIs) into MultiBacMam™ acceptor and donor vectors
  2. Match and mix your GOIs and then (re)combine them into one construct
  3. Select your construct using unique combination of antibiotic markers
  4. Transfer the entire GOIs-assembly into the MultiBacMam™ bacmid
  5. Select and amplify your gene-containing MultiBacMam™ bacmid
  6. Transfect insect cells with purified MultiBacMam™ bacmid
  7. Amplify baculovirus in insect cells and collect desired scale of virus
  8. Transfect mammalian cells with baculovirus
  9. Drug discovery, bioproduction, stem cell differentiation etc.
Baculovirus Based Gene Delivery Using MultiBacMam

MultiBacMam™. Recombinant baculoviruses are a useful tool for multi-gene transfer in mammalian cells.

Click A Reference to Download:

User Manual and Other Downloads

Click here to download MultiBacMam™ User Manual v3.0

Click here to download Cre-ACEMBLER User Manual v2.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows (32bit)

Click here to download Cre-ACEMBLER for Linux

Kits

MultiBacMam™ Expression System Kit 1,149.00€ ENQUIRY

Cell Lines

DH10EMBacVSV 819.00€ ENQUIRY
PirHC 279.00€ ENQUIRY
PirLC 279.00€ ENQUIRY

Plasmids

pACEMam1 (5µg) 279.00€ ENQUIRY
pACEMam2 (5µg) 279.00€ ENQUIRY
pMDC (5µg) 279.00€ ENQUIRY
pMDK (5µg) 279.00€ ENQUIRY
pMDS (5µg) 279.00€ ENQUIRY

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