BacMam is an increasingly popular system that facilitates delivery of heterologous DNAs to broad range of mammalian transduction targets. BacMam uses baculovirus as a vector, and has distinct advantages over other mammalian transfection methods given the large heterologous DNA carrying capacity of baculovirus (>100 kbp), the uptake of baculovirus by a broad range of mammalian cell types, and the sophisticated molecular biology technologies that have evolved over the last 20 years to support generation of baculovirus expression vectors.
MultiBacMam™ is the first BacMam system designed specifically to allow modular, DNA recombination based assembly of multiexpression cassettes for multiprotein co-expression in mammalian cells. MultiBacMam™ uses the same DNA recombination technologies as MultiBac™, but with mammalian promoters, and a "mammalianized" baculovirus- displaying a VSV peptide on the baculovirus surface that increases virus uptake by an order of magnitude, and a stably integrated fluorescent protein to simplify monitoring of experiments.
MultiBacMam™ transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle. The MultiBacMam™ baculovirus genome (DH10EMBacVSV) is under continuous development to remove harmful/non-essential genes (e.g. v-cath and chiA) and DNA recombination hotspots, and contains an integrated fluorescent protein expression cassette to allow for ease of monitoring virus titer. The MultiBacMam™ virus particle displays the VSV peptide on its surface to increase efficiency of entry into mammalian cells by around 1 order of magnitude compared to prior BacMam virus systems.
A specific subset of transfer vectors for use in primary cells called “MutiPrime” were developed by Mansouri et al 2016 (Nature Communications 2016, see reference download link below).
To advance the MultiPrime technology, Geneva Biotech and collaborators are developing a series of additional primary cell-specific transduction systems which specialize in tough to transduce cell types.
MultiBacMam™ vectors together with the MultiPrime vectors allows you to run the assays you need in the cellular models you want. This technology is first gene delivery system that easily allows for multiple DNA-encoded factors to be delivered on one vector into mammalian cells, tissues, or organisms. It is the first to allow expression of multi-component pathways (e.g. signal cascades), or complex targets such as multicomponent ion channels for drug discovery in mammalian cells tissues or organisms, all without the need for complicated multi vector co-transfections.
The first method for delivering a multigene readout into diverse mammalian and primary cells lines is described in Mansouri-et-al-2016-Nature-Communications (below link).
Click here to download MultiBacMam™ User Manual v4.7
Click here to see a list of MultiBacMam™ compatible cells
Click here for DNA vector sequences
Click here to download Cre-ACEMBLER User Manual 3.0
Click here to download Cre-ACEMBLER Short Description
