Understanding the quantitative functional consequences of human disease mutations requires silencing of endogenous genes and expression of mutants at close to physiological levels. Changing protein levels above or below these levels is also important for system perturbation and modelling. Fast design optimization demands flexible interchangeable cassettes for endogenous gene silencing and tuneable expression. ‘TEMTAC’ is a multigene recombineering and delivery system for simultaneous siRNA-based knockdown and regulated mutant (or other variant) expression with different dynamic ranges.
Click here to download Beltran-Sastre et al 2015. Nature Scientific Reports.
GENEVA BIOTECH CO-FOUNDERS PUBLISH IN SCIENCE GOOD REVIEW IN FORBES Geneva Biotech co-founders publish in Science good review in Forbes ...View all