The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary biological research. Coexpression of several proteins in the same mammalian cell is required for several critical areas of drug discovery in the pharmaceutical industry, such as heterologous expression of signaling pathways. Similarly, co-expression of multiple proteins in one cell is necessary to manipulate cell fate in stem cell biology. The capability to bring multiple heterologous genes into one cell is also a cornerstone of synthetic biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. MultiMam™ Stable is a novel and highly efficient modular plasmid-based expression system for generating multiprotein-expressing stable mammalian cell lines.
MultiMam™ Stable transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.