Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. Founded in 2012

The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary biological research. Coexpression of several proteins in the same mammalian cell is required for several critical areas of drug discovery in the pharmaceutical industry, such as heterologous expression of signaling pathways. Similarly, co-expression of multiple proteins in one cell is necessary to manipulate cell fate in stem cell biology. The capability to bring multiple heterologous genes into one cell is also a cornerstone of synthetic biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. MultiMam™ Stable is a novel and highly efficient modular plasmid-based expression system for generating multiprotein-expressing stable mammalian cell lines.

MultiMam™ Stable transfer vectors are fully synthetic, comprising only functional DNA, and as a result are small and easy to handle.

How MultiMam™ Stable Works:

• 1) Design and clone your gene(s) of interest (GOIs) into MultiMam™ Stable acceptor and donor vectors
• 2) Match and mix your GOIs and then (re)combine them into one construct
• 3) Select your construct using unique combination of antibiotic markers
• 4) Transfect mammalian cells with composite GOI vector.
• 5) Stable integration, gene amplification/selection. Stable expression.
• 6) Drug discovery, bioproduction, stem cell differentiation etc.

References and Downloads:

MultiMam Stable Manual v2.3

Click here to download Kriz et al 2010

Click here for DNA vector sequences

Click here to download Cre-ACEMBLER User Manual 3.0

Click here to download Cre-ACEMBLER Short Description

Click here to download Cre-ACEMBLER for Windows

Click here to download Cre-ACEMBLER for Linux